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Biosynthesis of Cholesterol, Steroids, and Isoprenoids. Cholesterol Is Made from Acetyl-CoA in Four Stages. Cholesterol, like long-chain fatty acids, is made from acetyl-CoA, but the assembly plan is quite different in the two cases. In early experiments animals were fed acetate labeled with. 1 Synthesis of Mevalonate from Acetate. The first stage in cholesterol biosynthesis leads to the intermediate mevalonate. These first two reactions, catalyzed by thiolase. In the next stage of cholesterol synthesis,.
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